Tau ELISA Kit Wako Technical Information and Data

Performance

Standard curve range	4.10 pg/mL - 1,000 pg/mL
Reactive Tau	Total Tau
Sample	Human cerebrospinal fluid (CSF)
Sample volume	10 µL～^※1
Assay time	3 hours
Detection method	Luminescent system^※2

※1: It is recommended that CSF sample volume is 50 μL, considering the accuracy of dilutions.
※2: A luminescence plate reader is required for the assay.

Principles

This kit is sandwich ELISA measuring phosphorylated Tau T181 by using 2 monoclonal antibodies. One recognizes phosphorylated Tau T181, and another reacts middle region of Tau.

Tau isoforms

Phosphorylation sites of Tau

Application data

Spike-and-recovery and dilution linearity

Spike-and-recovery

Human CSF 1	Not spiked	+ 20 pg/mL Tau		+ 60 pg/mL Tau		+ 150 pg/mL Tau
	Measured	Measured	Recovery	Measured	Recovery	Measured	Recovery
	4.09 pg/mL	24.8 pg/mL	104％	58.1 pg/mL	90％	175 pg/mL	114％
Human CSF 2	Not spiked	+ 40 pg/mL Tau		+ 135 pg/mL Tau		+ 380 pg/mL Tau
	Measured	Measured	Recovery	Measured	Recovery	Measured	Recovery
	24.8 pg/mL	70.5 pg/mL	114％	160 pg/mL	100％	390 pg/mL	96.1％

Dilution linearity

These assays demonstrated that the kit is highly quantitative.

Measurements of human CSF

Cerebrospinal fluids(CSF) from patients were measured with our ELISA kit and competitor’s kit.
The samples are from Alzheimer disease (AD) , mild cognitive impairment(MCI) and non-dementia patients.

The differences between non-dementia, MCI, and AD groups were more significant when measured with our kit.

Correlation with Mini Mental State Examination (MMSE) scores

※MMSE score
　≤ 23: suspected of dementia
　24-27: suspected of mild cognitive impairment
　28-30: normal

The correlation between MMSE scores and the concentration of Tau was examined. Samples were collected from CSF and measured using our ELISA kit.

There was a correlation between the concentration of Tau and MMSE score. The correlation was stronger when Tau concentration was measured using our product.