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Preparation: DNA is extracted from T4 phage purified by a CsCl-density gradient centrifugation from T4 phage-dC (+) infected E. coli MC 1061
Solution in 10 mmol/L Tris-HCl (pH 7.9) and 1mmol/L EDTA
Concentration: 0.2 ~ 0.5 ug/uL
Purity: A260/ A280 = approx. 1.8
Note: DNA contains cytosine in place of Hydroxymethyl cytosine.
References:
Takahashi, H., Saito, H. and Ikeda, Y.: Agric. Biol. Chem., 43,841 (1979).
Takahashi, H., Shimizu, M., Saito, H., Ikeda Y. and Sukisaki, H.: Gene,5, 9 (1979).
Takahashi, H., Shimizu, M., Saito, H. and Ikeda, Y.: Mol. Gen. Genet.,168, 49 (1979).
Takahashi, H. and Saito, H.: Plasmid, 8, 29 (1982).
Manufacturer:
Nippon Gene Co., Ltd.
Made by FUJIFILM Wako Pure Chemical Corporation and distributed by FUJIFILM Irvine Scientific.
For research use only. Not for use in diagnostic procedures.
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