Recombinant Human Noggin

Human Noggin (h Noggin, NOG) is a secreted homodimeric glycoprotein and is best known for inducing the development of neural tissue and inhibiting bone morphogenetic proteins (BMPs) such as BMP-4 (1,2). h Noggin inhibits BMPs by binding to BMP epitopes required for binding their Type I and Type II receptors, thus mediating a pathway that controls processes such as the establishment of the embryonic dorsal–ventral axis, induction of neural tissue, formation of joints in the skeletal system and neurogenesis in the adult brain (3,4).

Human Noggin has also proven to be useful in the culturing of human embryonic stem cells (hESC). The addition of human Noggin to hESC culture in unconditioned media synergizes with basic fibroblast growth factor (bFGF) and inhibits hBMP4 activity, thus allowing the hESCs proliferation while maintaining their undifferentiated state (4,5). In preclinical models, hNoggin has a high expression after induction of spinal cord injury and may play an important neuroprotective role in spinal cord injuries (6).

Product images on the website are not representative of actual product, including, but not limited to differences in cap color, tube type, or label format.

Instructions

Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Technical Specifications

*Lot-specific values for the above specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

References:

1. DM Valenzuela, AN Economides, E Rojas, TM Lamb, L Nunez, P Jones, NY Lp, R Espinosa 3rd, CI Brannan, DJ Gilbert. Journal of Neuroscience 1 September 1995, 15 (9) 6077-6084.
2. Carola Krause, Asja Guzman, Petra Knaus, Noggin, The International Journal of Biochemistry & Cell Biology, Volume 43, Issue 4, 2011, Pages 478-481, ISSN 1357-2725.
3. Lisa J. Brunet et al., Noggin, Cartilage Morphogenesis, and Joint Formation in the Mammalian Skeleton.Science280,1455-1457(1998).
4. Groppe, J., Greenwald, J., Wiater, E. et al. Structural basis of BMP signalling inhibition by the cystine knot protein Noggin. Nature 420, 636–642 (2002).
5. Xu, RH., Peck, R., Li, D. et al. Basic FGF and suppression of BMP signaling sustain undifferentiated proliferation of human ES cells. Nat Methods 2, 185–190 (2005).
6. Al-Sammarraie, Nadia; Mahmood, Mohammed; Ray, Swapan K.*. Neuroprotective role of Noggin in spinal cord injury. Neural Regeneration Research 18(3): p 492-496, March 2023.