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CTGrade GMP rh PDGF-BB is a recombinant human protein that is produced from E. coli and is designed to support preclinical and clinical research, as well as commercial applications, and offers:
Size: 50 µg, 100 µg, and 1 mg. Custom sizes and packaging available on request.
PDGF-BB (Platelet-derived growth factor) comprises a family of homo or heterodimeric growth factors, including PDGF-AA, PDGF-BB, PDGF-CC, and PDGF-DD.
The biological activity of CTGrade GMP interleukins and growth factors is standardized, where applicable, to WHO International standards, providing cell and gene therapy developers consistent, lot-to-lot biological activity and performance.
The CTGrade GMP products are manufactured in a facility that does not use or process beta-lactam containing materials. No animal- or human-derived materials were used during manufacturing or as ingredients. These products are manufactured, tested, and released in an ISO 9001:2015 certified facility following cGMP practices. USP chapter <1043> for ancillary materials has been considered in the manufacture of these products.
Figure 1. CTGrade GMP rh PDGF-BB (1 μg) was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by InstantBlue staining showing a single prominent band at 12.4 kDa (R) and 24.9 kDa (NR).*
*Predicted MW is 12.4 kDa and 24.9 kDa, but runs on SDS-PAGE at ~15 kDa (R) and ~30 kDa (NR).
Figure 2. The biological activity of CTGrade GMP rh PDGF-BB was determined in a NR6R/3T3 cell-based proliferation assay.
Alternate Names | GDGF, ODGF |
Accession Number | P01127 |
AA Sequence | MSLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETVAAARPVT |
Predicted Molecular Mass | Dimer, 12.4/24.9 kDa (22/110 aa) |
Formulation | CTGrade GMP rh PDGF-BB lyophilized at 1 mg/ml in 10 mM Sodium Phosphate, pH 7.5, 0.2 µm filtered |
Protein Purity | ≥ 98% determined with Coomassie staining |
Endotoxin | < 0.01 EU/µg using USP <85> / EP 2.6.14 |
Bioactivity | ED50 is determined by the dose-dependent proliferation of NR6R-3T3 cells. The ED50 is typically less than 5 ng/mL. The international units of CTGrade GMP rh PDGF-BB is approximately 1.6 x 103 IU/µg, which is calibrated against recombinant human platelet-derived growth factor BB WHO International Standard (NIBSC code 94/728). |
Quality | Carrier-free and no animal- or human-derived materials were used during manufacturing |