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Nerve growth factor beta (β-NGF) is a neurotrophic factor that is important for the development and maintenance of sensory and sympathetic neurons. β-NGF signals through the low affinity nerve growth factor receptor (LNGFR) and the tropomyosin receptor kinase A (TrkA) to activate PI3K, Ras, and PLC signaling pathways. β-NGF is also involved in the growth, differentiation, and survival of B lymphocytes. Human, mouse, and rat β-NGF proteins are cross-reactive.
Product images on the website are not representative of actual product, including, but not limited to differences in cap color, tube type, or label format.
SDS-PAGE Showing a Single Prominent Band at 13.6 kDa
Figure 1. rm β-NGF (1μg) was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by Instant Blue staining showing a single prominent band at 13.6 kDa. Expected MW is 13.6/27.2 kDa but runs on SDS-PAGE at 13.6 kDa
Cell-Based Proliferation Assay
Figure 2. Recombinant Mouse β-NGF induced proliferation in TF-1 cells.
Instructions
Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
Lyophilized from a 0.2 µm filtered solution containing 0.1% Trifluoroacetic Acid (TFA)
Product Reconstitution
Sterile water at 0.1 mg/mL
Country of Origin
USA
Specification*
Method of Determination
Acceptance Criteria
Purity
Reducing and Non-Reducing SDS PAGE
≥ 95%
Endotoxin
Kinetic LAL
≤ 0.1 EU/µg
Biological Activity (ED50)
TF-1 cell proliferation
≤ 5 ng/mL
*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.
beta-NGF beta NGF
Specifications
Storage
At or below -20°C
Shelf Life
12 months from date of receipt when stored at -20°C to-80°C as supplied.
