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IL-12 is a heterodimeric cytokine, encoded by IL-12A and IL-12B genes, responsible for the p35 and p40 subunits respectively. It is produced by macrophages and B lymphocytes and has a broad range of effects, including the activation of T cells and Natural Killer (NK) cells (1,2). A key effect includes inducing production of IFN-γ and TNF-α by resting and activating T and NK cells through STAT4 pathway which represents a critical path for T cell-dependent induction of IFN-γ (3). This function is necessary for fighting bacterial and parasitic infections, activation of differentiated T lymphocytes, and development of Th1 response. hIL-12 is therefore understood to be a critical link between innate and adaptive immune responses (3,4).
Product images on the website are not representative of actual product, including, but not limited to differences in cap color, tube type, or label format.
SDS-PAGE Showing a Single Prominent Band at 72 kDa
Figure 1. Western Blot image showing detection of rh IL-12 bands
Cell-based Proliferation Assay
Figure 2. Recombinant Human IL-12 activity induces bioluminiscence upon binding to receptor on IL-12 BioAssay Cells
Bioactivity Comparison with Commercially-available rh IL-12
Figure 3. Recombinant Human IL-12 activity induces bioluminiscence upon binding to receptor on IL-12 BioAssay Cells
Instructions
Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
Lyophilized from a 0.2 µm filtered solution containing 10 mM NaP, 30 mM NaCl, pH 7.2
Product Reconstitution
Sterile water at 0.1 mg/mL
Country of Origin
USA
Specification*
Method of Determination
Acceptance Criteria
Purity
Reducing and Non-Reducing SDS PAGE
≥ 98%
Endotoxin
Kinetic LAL (50% confidence)
≤ 0.1 EU/µg
Biological Activity (ED50)
Ability to induce Bioluminescence in engineered IL-12 bioassay cells
≤ 2.5 ng/mL; 4.0 x 105 units/mg
*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.
