Phosphorylated Tau T181 ELISA Kit Wako Technical Information and Data

Performance

Range	4.40 - 500 pg/mL
Specifity	Phosphorylated Tau T181
Sample	Human cerebrospinal fluid (CSF)*
Sample volume	20 μL
Assay time	Approx. 20 hours
Detection method	Luminescence method**

* Not recommended for plasma and serum.
** Requires luminescence plate reader for measurement.

Assay principle

This kit is sandwich ELISA measuring phosphorylated Tau T181 by using 2 monoclonal antibodies. One recognizes phosphorylated Tau T181, and another reacts middle region of Tau.

Measurement example

Spike-recovery test

Human CSF1	Not spiked	＋20pg/mL pTau		＋100pg/mL pTau		＋200 pg/mL pTau
	Measured	Measured	Recovery	Measured	Recovery	Measured	Recovery
	53.7pg/mL	73.4pg/mL	98.5%	147pg/mL	93.3%	271pg/mL	109%
Human CSF2	Not spiked	＋20pg/mL pTau		＋100pg/mL pTau		＋200 pg/mL pTau
	Measured	Measured	Recovery	Measured	Recovery	Measured	Recovery
	14.0pg/mL	32.8pg/mL	94.0％	107pg/mL	93.0％	225pg/mL	106%

Linearity of dilution test

In each test, this product showed high quantitativeness.

Human CSF measurements at different dementia stages

Cerebrospinal fluids(CSF) from patients were measured with our ELISA kit and competitor’s kit.
The samples are from Alzheimer disease (AD) , mild cognitive impairment(MCI) and non-dementia patients.

	MMSE Score	Wako	Company A	Company B
Non-AD1	30	22.8	12.1	15.4
Non-AD2	22	14.6	22.8	ND
Non-AD3	29	9.7	13.2	ND
Non-AD4	30	17.2	27	2.48
Non-AD5	30	24.8	33.5	0.88
MCI1	26	23.5	37.8	ND
MCI2	23	67.7	85.6	0.58
MCI3	23	38.4	38.9	ND
MCI4	24	ND	ND	ND
MCI5	25	63.6	77.2	ND
AD1	16	35.2	73.4	5.58
AD2	19	36.2	21.1	ND
AD3	14	193	226	17.2
AD4	24	75	46.1	ND
AD5	10	204	184	9.81

Red: Low reliability because of not detected or out of standard curve.

A significant difference observed between non-dementia, MCI and Alzheimer's disease.

Correlation between Tau pT181 concentration and MMSE score

MMSE score

Diagnosis Inquiry test score of cognitive function
30-28: Normal
27-24: Suspected of mild cognitive impairment
≦ 23: Suspected of dementia

The high correlation was observed between Tau pT181 concentration and MMSE score.