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Range | 4.40 - 500 pg/mL |
---|---|
Specifity | Phosphorylated Tau T181 |
Sample | Human cerebrospinal fluid (CSF)* |
Sample volume | 20 μL |
Assay time | Approx. 20 hours |
Detection method | Luminescence method** |
* Not recommended for plasma and serum.
** Requires luminescence plate reader for measurement.
This kit is sandwich ELISA measuring phosphorylated Tau T181 by using 2 monoclonal antibodies. One recognizes phosphorylated Tau T181, and another reacts middle region of Tau.
Human CSF1
|
Not spiked
|
+20pg/mL pTau
|
+100pg/mL pTau
|
+200 pg/mL pTau
|
|||
---|---|---|---|---|---|---|---|
Measured
|
Measured
|
Recovery
|
Measured
|
Recovery
|
Measured
|
Recovery
|
|
53.7pg/mL
|
73.4pg/mL
|
98.5%
|
147pg/mL
|
93.3%
|
271pg/mL
|
109%
|
|
Human CSF2
|
Not spiked
|
+20pg/mL pTau
|
+100pg/mL pTau
|
+200 pg/mL pTau
|
|||
Measured
|
Measured
|
Recovery
|
Measured
|
Recovery
|
Measured
|
Recovery
|
|
14.0pg/mL
|
32.8pg/mL
|
94.0%
|
107pg/mL
|
93.0%
|
225pg/mL
|
106%
|
In each test, this product showed high quantitativeness.
Cerebrospinal fluids(CSF) from patients were measured with our ELISA kit and competitor’s kit.
The samples are from Alzheimer disease (AD) , mild cognitive impairment(MCI) and non-dementia patients.
MMSE Score | Wako | Company A | Company B | |
---|---|---|---|---|
Non-AD1 | 30 | 22.8 | 12.1 | 15.4 |
Non-AD2 | 22 | 14.6 | 22.8 | ND |
Non-AD3 | 29 | 9.7 | 13.2 | ND |
Non-AD4 | 30 | 17.2 | 27 | 2.48 |
Non-AD5 | 30 | 24.8 | 33.5 | 0.88 |
MCI1 | 26 | 23.5 | 37.8 | ND |
MCI2 | 23 | 67.7 | 85.6 | 0.58 |
MCI3 | 23 | 38.4 | 38.9 | ND |
MCI4 | 24 | ND | ND | ND |
MCI5 | 25 | 63.6 | 77.2 | ND |
AD1 | 16 | 35.2 | 73.4 | 5.58 |
AD2 | 19 | 36.2 | 21.1 | ND |
AD3 | 14 | 193 | 226 | 17.2 |
AD4 | 24 | 75 | 46.1 | ND |
AD5 | 10 | 204 | 184 | 9.81 |
Red: Low reliability because of not detected or out of standard curve.
A significant difference observed between non-dementia, MCI and Alzheimer's disease.
MMSE score
Diagnosis Inquiry test score of cognitive function
30-28: Normal
27-24: Suspected of mild cognitive impairment
≦ 23: Suspected of dementia
The high correlation was observed between Tau pT181 concentration and MMSE score.