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Mature BDNF ELISA Kit Wako Data
Performance
Standard curve
| Standard curve range | 4.1 - 1,000 pg/mL |
|---|---|
| Specificity | mBDNF* |
| Sample | Human serum and plasma** |
| Sample volume | Human serum：10 μL Human plasma: 5 μL |
| Assay time | 4 hours |
| Detection method | Colorimetric method |
* Approximately 10% reaction to proBDNF
** This antibody can also detect mouse and rat BDNF.
Assay principle
This kit is a sandwich ELISA using mBDNF monoclonal antibody and mBDNF polyclonal antibody. The kit has low cross-reactivity to proBDNF(approx. 10%) because the monoclonal antibody reacts with N-terminal cutting surface of mBDNF.
| Fujifilm Wako | Company A | Company B | Company C | |
|---|---|---|---|---|
| Cross-reactivity to proBDNF | Approx. 10％ | Approx. 10％ | Approx. 15％ | Approx. 50％ |
| Minimum concentration of assay range |
4.1 pg/mL | 62.5 pg/mL | 15.6 pg/mL | 15.0 pg/mL |
Data
Spiked recovery test
| Sample | Spike mBDNF (pg/mL) | Assay value (pg/mL) | Recovery volume (pg/mL) | Recovery (%) |
|---|---|---|---|---|
| Human serum ① |
0 | 330 | - | - |
| 100 | 423 | 93.0 | 93.0 | |
| 250 | 581 | 251 | 100 | |
| 500 | 817 | 487 | 97.4 | |
| Human serum ② |
0 | 56.6 | - | - |
| 50 | 106 | 49.4 | 98.8 | |
| 100 | 154 | 97.4 | 97.4 | |
| 200 | 257 | 200 | 100 | |
| 300 | 363 | 306 | 102 |
| Sample | Spike mBDNF (pg/mL) | Assay value (pg/mL) | Recovery volume (pg/mL) | Recovery (%) |
|---|---|---|---|---|
| Human plasma ① |
0 | 156 | - | - |
| 100 | 252 | 96.0 | 96.0 | |
| 250 | 407 | 251 | 100 | |
| 500 | 661 | 505 | 101 | |
| Human plasma ② |
0 | 66.0 | - | - |
| 50 | 115 | 49.0 | 98.0 | |
| 100 | 164 | 98.0 | 98.0 | |
| 200 | 276 | 210 | 105 | |
| 300 | 369 | 303 | 101 |
Dilution linearity
Example of measurement -clinical sample -
Their serum mBDNF levels were measured by this kit. The samples were from healthy controls and patients with major depression, bipolar disorder, or schizophrenia.
Healthy control (n=6)
| No. | Sex | Age | Assay value (ng/mL) |
|---|---|---|---|
| 1 | F | 48 | 14.5 |
| 2 | F | 50 | 22.8 |
| 3 | F | 54 | 20 |
| 4 | F | 47 | 18.5 |
| 5 | F | 42 | 13.8 |
| 6 | F | 60 | 19.1 |
Major depression (n=6)
| No. | Sex | Age | Assay value (ng/mL) |
|---|---|---|---|
| 1 | F | 66 | 15.7 |
| 2 | F | 38 | 18.1 |
| 3 | F | 43 | 10.0 |
| 4 | F | 52 | 16.6 |
| 5 | F | 41 | 22.3 |
| 6 | F | 58 | 15.1 |
Bipolar disorder (n=4)
| No. | Sex | Age | Assay value (ng/mL) |
|---|---|---|---|
| 1 | F | 46 | 16.5 |
| 2 | F | 52 | 20.9 |
| 3 | F | 52 | 11.5 |
| 4 | F | 52 | 11.5 |
Schizophrenia (n=4)
| No. | Sex | Age | Assay value (ng/mL) |
|---|---|---|---|
| 1 | F | 36 | 17.9 |
| 2 | F | 53 | 23 |
| 3 | F | 55 | 14.6 |
| 4 | F | 51 | 10.8 |
| Healthy person | Major depression | Bipolar disorder | Schizophrenia |
| 18.1 ± 3.41 | 16.3 ± 4.02 | 15.1 ± 4.53 | 16.6 ± 5.17 |
(ng/mL)
