Q: Is it OK to use for RNA precipitation?

A: It is OK. It can be used to recover RNA.

Q: Are there any DNase or RNase contaminants?

A: No. Ethachinmate has been tested and confirmed to be DNase-free and RNase-free.

Q: Which length and concentration of DNA and RNA are recovered?

A: It is possible to recover almost all of the DNA (> 100 base pairs) and RNA (> 120 bases). If the concentration of nucleotide is less than 20 ng/ml, efficiency may lower.

Q: Does it have any effect on quantitation by absorbance at 260 nm?

A: No. It does not affect quantitation by A260 measurement.

Q: My DNA solution is less than 100 µl. How much Ethachinmate should I use?

A: Add 1 µl of Ethachinmate. Making the DNA pellet visible requires over 1 µl of Ethachinmate. Calculate the amount of 3 mol/l Sodium Acetate to add into your DNA solution based on its volume. (e. g., for 50 µl of DNA solution, add 1 µl of Ethachinmate and 1.7 µl of 3 mol/l Sodium Acetate).

Q: How much Ethachinmate should I use if the DNA solution volume is more than 300 µL?

A: 3 µL of Ethachinmate is sufficient even for larger volumes. Do not add more than this. Adjust sodium acetate proportionally (e.g., for 600 µL DNA solution, add 3 µL Ethachinmate and 19.8 µL of 3 M sodium acetate).

Q: I want to perform ethanol precipitation two or more times. Should I add Ethachinmate for each precipitation?

A: No. Once you add Ethachinmate into DNA solution there is no need to add Ethachinmate again. Multiple additions of Ethachinmate may make the solution viscous, causing subsequent processes to become more difficult.

Q: Does freezing affect quality of Ethachinmate?

A: No, it doesn't affect the quality.

Q: Does autoclave freeze affect quality of Ethachinmate?

A: No, it doesn't affect the quality.

Q: Does phenol / chloroform treatment of DNA solution containing Ethachinmate bring any effect on the quality of Ethachinmate?

A: No, it doesn't affect the quality.

Q: Does using Ethachinmate affect band pattern of gel electrophoresis?

A: No. However, depending on the electrophoresis conditions, DNA bands larger than several tens of kbp may appear broad.

Q: Does Ethachinmate affect digestion using restriction enzyme?

A: No, it doesn't affect digestion.

Q: Does Ethachinmate affect ligation using T4 DNA ligase?

A: No, it doesn't affect ligation.

Q: Does Ethachinmate affect cDNA synthesis using AMV reverse transcriptase?

A: No, it doesn't affect cDNA synthesis.

Q: Does Ethachinmate affect PCR using Taq DNA polymerase?

A: No, it doesn't affect PCR.

Q: Does Ethachinmate affect reaction of Klenow Fragment?

A: No, it doesn't affect.

Q: Does Ethachinmate affect transformation of E. coli?

A: No. It does not affect electroporation either. However, please note that when Ethachinmate, PEG6000, or polyamines are present in a ligation product solution to be introduced into E. coli, the transformation efficiency may decrease.

Q: Does Ethachinmate affect in vitro Packaging?

A: It slightly decreases the efficiency of lambda packaging.

Q: Does Ethachinmate affect transfection efficiency?

A: When siRNA prepared using Ethachinmate was transfected with a lipofection reagent, a decrease in efficiency was observed. Although other transfection methods have not been tested, Ethachinmate may reduce transfection efficiency.

Q: Does ethanol precipitation with Ethachinmate precipitate mono nucleotide?

A: Experiments using 8 and 17 base nucleotides did not show significant differences between the presence or the absence of Ethachinmate.

Q: Does Ethachinmate get denatured in hybridization solution containing formamide?

A: No. It is not become denatured.

Q: Does Ethachinmate affect blotting?

A: No, it doesn't affect blotting

Q: Does Ethachinmate affect sequencing?

A: It does not affect cycle sequencing with ABI Prism 377, or sequencing by dideoxy method.

Q: Does the addition of Ethachinmate make the pellet more likely to detach from the tube?

A: It depends on the type of material of the tube. (e. g., "Safe-Lock tube" from Eppendorf tends to show this.)

Q: Can nucleic acids containing Etachinmate be used for Agilent Bioanalyzer analysis?

A: When we analyzed RNA samples that contained an estimated 0.1 %–0.5 % carryover of Ethachinmate, no particular problems were observed. (This observation was made using the Agilent RNA 6000 Pico Kit; the effects on other Bioanalyzer kits have not been confirmed.)