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Y-27632 is selective and potent ROCK inhibitor. Y-27632 plays various roles including contraction of vascular smooth muscle cells via ROCK signaling pathways. Furthermore, it suppresses the cell death of human embryonic stem cells (hESCs) and human induced Pluripotent Stem Cells (hiPSCs) at cell dispersion, and that it increases the survival rate of hESCs and hiPSCs during cryopreservation.
For Cell Culture
Manufacturer: | FUJIFILM Wako Pure Chemical Corporation |
Specification Assay: | 98.0+% (HPLC) |
CAS RN: | 331752-47-7 |
Molecular Formula: | C14H21N3O・2HCl・H2O |
Molecular Weight: | 338.27 |
Precautions for Use: | Packed on inactive gas |
Appearance: | White - pale yellow, crystalline powder - powder |
Boiling Point: | about 85 degrees C |
Specific Rotation: | +2 - +10 degrees (D/20 degrees C)(c=0.5,CH3OH) |
Purity: | Bacterial endotoxins : < 0.25 EU/mg |
Alias: | (R)-(+)-trans-N-(4-pyridyl)-4-(1-aminoethyl)-cyclohexanecarboxamide Dihydrochloride Monohydrate |
Solubility Data for Y-27632 dihydrochloride
C14H21N3O・2HCl・H2O = 338.27
Solvent | Conc. mg/mL | Conc. mmol/L | |
Solubility | Water | 10 mg/mL | 29.56 mmol/L |
Ethanol | 10 mg/mL | 29.56 mmol/L |
C14H21N3O・2HCl・H2O = 338.27
Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
1 mmol/L | 2.956 mL | 14.781 mL | 29.562 mL |
5 mmol/L | 0.591 mL | 2.956 mL | 5.912 mL |
10 mmol/L | 0.296 mL | 1.478 mL | 2.956 mL |
50 mmol/L | 0.059 mL | 0.296 mL | 0.591 mL |
ICH-Q7 | ISO9001 | Sterility test |
Viable count test |
Animal- derived- free |
Bacterial Endotoxin test |
Mycoplasma test |
Powder/ Liquid |
References* | |
Y-27632 (GMP-compliant)** | ✓ | ✓ | - | ✓ | ✓ | ✓ | ✓ | Powder | - |
Y-27632, MF*** | - | ✓ | - | ✓ | ✓ | ✓ | ✓ | Powder | Citations |
CultureSure Y-27632 | - | ✓ | - | - | ✓ | ✓ | ✓ | Powder | Citations |
CultureSure 10mmol/L Y-27632 Solution, Animal-derived-free |
- | ✓ | ✓ | - | ✓ | ✓ | ✓ |
Liquid Sterilized with filter |
* Click to move to the PubMed Central search results.
** This product is complied with ICH-Q7 (GMP for APIs) as a raw material for commercial production of regenerative medicine products.
*** The manufacturing process and analytical procedure are validated.
Made by FUJIFILM Wako Pure Chemical Corporation and distributed by FUJIFILM Irvine Scientific.