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Shenandoah CTGrade GMP rh IL-6 is a recombinant human protein that is produced from E. coli and is designed to support basic, translational, and clinical research, as well as commercial applications.
Size: 50 µg, 100 µg, and 1 mg. Custom sizes and packaging available on request.
IL-6 is an important pro-inflammatory and anti-inflammatory cytokine expressed by T cells, macrophages, and muscle cells.
The biological activity of CTGrade GMP interleukins and growth factors is standardized, where applicable, to WHO International standards, providing cell and gene therapy developers consistent, lot-to-lot biological activity and performance.
The CTGrade GMP products are manufactured in a facility that does not use or process beta- lactam containing materials. No animal- or human-derived materials were used during manufacturing or as ingredients. These products are manufactured, tested, and released in an ISO 9001:2015 certified facility following GMP. USP chapter <1043> for ancillary materials has been considered in the manufacture of these products.
Figure 1. CTGrade GMP rh IL-6 (1 μg) was resolved with SDS-PAGE under non-reducing (NR) conditions and visualized by InstantBlue staining showing a single prominent band at 20.9 kDa.
Figure 2. The biological activity of CTGrade GMP rh IL-6 was determined by its ability to stimulate the proliferation of B9 cells.
Figure 3. CTGrade GMP rh IL-6 induces proliferation of B9 cells that is consistent between lots.
Figure 4. Bioactivity comparison between CTGrade GMP rh IL-6 and commercially-available GMP IL-6.
Alternate Names | IFN-β2, B-cell Differentiation Factor, BSF-2, HSF, MGI-2 |
Accession Number | P05231 |
AA Sequence | MPVPPGEDSK DVAAPHRQPL TSSERIDKQI RYILDGISAL RKETCNKSNM CESSKEALAE NNLNLPKMAE KDGCFQSGFN EETCLVKIIT GLLEFEVYLE YLQNRFESSE EQARAVQMST KVLIQFLQKK AKNLDAITTP DPTTNASLLT KLQAQNQWLQ DMTTHLILRS FKEFLQSSLR ALRQM |
Predicted Molecular Mass | Monomer, 20.9 kDa (185aa) |
Formulation | CTGrade GMP rh IL-6 lyophilized at 1 mg/ml in 10 mM Sodium Phosphate, pH 7.5, 0.2 µm filtered |
Protein Purity | ≥ 97% determined by reducing and non-reducing SDS-PAGE analysis |
Endotoxin | < 0.05 EU/µg using USP <85> / EP 2.6.14 |
Bioactivity | ED50 is determined by the dose-dependent proliferation of B9 cells. The ED50 is typically less than 0.025 ng/mL. The international units of CTGrade GMP rh IL-6 is approximately 1.2 x105 IU/µg, which is calibrated against recombinant human interleukin 6 WHO International Standard (NIBSC code 89/548). |
Quality | Carrier-free and no animal- or human-derived materials were used during manufacturing |
*For research use or further manufacturing and in ex vivo cell therapy applications.
**Animal component-free to the tertiary level (no animal-origin components or sub-components are used in the product, raw materials, including packaging or the manufacturing process). Supporting documentation available upon request.
***Lot-specific values for the following specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.