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Shenandoah CTGrade GMP rh IL-2C126S is a recombinant human protein that is produced from E. coli and is designed to support basic, translational, and clinical research, as well as commercial applications.
Size: 50 µg, 100 µg, and 1 mg. Custom sizes and packaging available on request.
IL-2C126S is an immunomodulatory cytokine that is produced by lymphocytes. IL-2C126S signals through the IL-2R receptor to induce activated T cell proliferation and promote T cell differentiation.
The biological activity of CTGrade GMP interleukins and growth factors is standardized, where applicable, to WHO International standards, providing cell and gene therapy developers consistent, lot-to-lot biological activity and performance.
The CTGrade GMP products are manufactured in a facility that does not use or process beta lactam containing materials. No animal- or human-derived materials were used during manufacturing or as ingredients. These products are manufactured, tested, and released in an ISO 9001:2015 certified facility following GMP. USP chapter <1043> for ancillary materials has been considered in the manufacture of these products.
Figure 1. CTGrade GMP rh IL-2C126S (1 μg) was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by InstantBlue staining showing a single prominent band at 14 kDa. Predicted MW is 15.5 kDa, but runs on SDS-PAGE at ~14kDa.
Figure 2. The biological activity of CTGrade GMP rh IL-2C126S was determined in a CTLL-2 cell-based proliferation assay.
Figure 3. CTGrade GMP rh IL-2C126S induces proliferation in CTLL-2 cells that is consistent between lots.
Figure 4. Bioactivity comparison between CTGrade GMP rh IL-2C126S and RUO rh IL-2C126S.
Figure 5. Bioactivity comparison between CTGrade GMP rh IL-2C126S and commercially-available GMP IL-2.
Alternate Names | T cell growth factor, TCGF, Aldesleukin |
Accession Number | P60568 |
AA Sequence | MAPTSSSTKK TQLQLEHLLL DLQMILNGIN NYKNPKLTRM LTFKFYMPKK ATELKHLQCL EEELKPLEEV LNLAQSKNFH LRPRDLISNI NVIVLELKGS ETTFMCEYAD ETATIVEFLN RWITFSQSII STLT |
Predicted Molecular Mass | Monomer, 15.5 kDa (134 aa) |
Formulation | CTGrade GMP rh IL-2C126S lyophilized at 1 mg/ml in 0.1% Trifluoroacetic Acid (TFA), 0.2 µm filtered |
Protein Purity | ≥ 97% determined by reducing and non-reducing SDS-PAGE analysis |
Endotoxin | < 0.01 EU/µg using USP <85> / EP 2.6.14 |
Bioactivity | ED50 is determined by the dose-dependent proliferation of CTLL- 2 cells. The ED50 is typically less than 3 ng/mL. The international units of CTGrade GMP rh IL-2C126S is approximately 1.17 x 104 IU/µg, which is calibrated against recombinant human interleukin 2 WHO International Standard (NIBSC code 86/500). |
Quality | Carrier-free and no animal- or human-derived materials were used during manufacturing |
*Animal component-free to the tertiary level (no animal components or sub-components are used in the product, raw materials, including packaging or the manufacturing process). Supporting documentation available upon request.
**For research use or further manufacturing and in ex vivo cell therapy applications.
***Lot-specific values for the following specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.