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Shenandoah CTGrade GMP rh IGF-1 is a recombinant human protein produced from E. coli and is designed to support preclinical and clinical research, as well as commercial applications.
High biological activity verified by a relevant bioactivity assay
Low endotoxin levels
≥ 98% purity
High lot-to-lot consistency
Size: 50 µg, 100 µg, and 1 mg. Custom sizes and packaging available on request.
Insulin-like Growth Factor 1 (IGF-1), also known as somatomedin C, is the principal effector of growth hormone and is structurally and functionally related to insulin, but with significantly stronger growth-promoting activity.
Plays essential roles in cell proliferation, migration, and differentiation in muscle regeneration and bone growth
Leveraged in oncology and neurodegenerative disease therapy, along with IGF-1 pathways, as active areas of investigation
Approved for the treatment of severe primary IGF-1 deficiency, such as Laron syndrome, or in children with growth hormone deficiency who develop anti-GH antibodies
The biological activity of CTGrade GMP interleukins and growth factors is standardized, where applicable, to WHO International standards, providing cell and gene therapy developers consistent, lot-to-lot biological activity and performance.
CTGrade GMP products are manufactured in a facility that does not use or process beta- lactam containing materials. No animal- or human-derived materials were used during manufacturing or as ingredients. These products are manufactured, tested, and released in an ISO 9001:2015 certified facility following GMP. USP chapter <1043> for ancillary materials has been considered in the manufacture of these products.
SDS-PAGE Showing a Single Prominent Band at 7.7 kDa
Figure 1. CTGrade GMP rh IGF-1 (1 μg) was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by Instant Blue staining showing a single prominent band at 7.7 kDa.
Cell-based Proliferation Assay
Figure 2. The biological activity of CTGrade GMP rh IGF-1 was determined in a cell proliferation assay using MCF-7 cells.
Lot-to-lot Consistency
Figure 3. CTGrade GMP rh IGF-1 induces proliferation in MCF-7 cells that is consistent between lots.
Bioactivity Comparison Between CTGrade GMP rh IGF-1 and Commercially-available rh IGF-1
Figure 4. Bioactivity comparison between CTGrade GMP rh IGF-1 and commercially-available rh IGF-1.
Technical Specifications
Formulation
CTGrade GMP rh IGF-1 is lyophilized from a 0.2 μm filtered solution containing 0.1% Trifluoroacetic Acid (TFA)
Protein Purity
≥ 98% determined by reducing and non-reducing SDS-PAGE analysis
Endotoxin
< 0.01 EU/μg using USP <85> / EP 2.6.14
Bioactivity
ED50 is determined by the dose-dependent MCF-7 cell line cells. ED50 is typically less than 1.5 ng/mL.
Quality
Carrier-free and no animal- or human-derived materials were used during manufacturing
Specifications
Storage
This product is shipped at ambient temperature. Immediately upon receipt, store the lyophilized protein at -20°C in a manual defrost freezer.
Shelf Life
Unopened vials are stable for 36 months from the date of manufacture. Reconstituted material should be apportioned in working volumes and stored at or below -20°C in manual defrost freezer. For short-term storage, reconstituted material is stable for 4-6 weeks when stored at 2-8°C. Stability can be increased by adding at least 0.1% carrier protein.
