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Shenandoah CTGrade GMP rh IFN-gamma is a recombinant human protein produced from E. coli and is designed to support basic, translational, and clinical research, as well as commercial applications.
High biological activity verified by a relevant bioactivity assay
Low endotoxin levels
≥ 98% purity
High lot-to-lot consistency
Size: 50 µg, 100 µg, and 1 mg. Custom sizes and packaging available on request.
Interferon-gamma (IFN-γ), also known as type II or immune interferon, is a macrophage-activating cytokine with broad immune-regulatory functions and is considered the prototype pro-inflammatory cytokine. It is primarily produced by T lymphocytes and natural killer (NK) cells in response to antigens, mitogens, and other cytokines.
Induces class I and II MHC molecules, activates macrophages and monocytes
Promotes Th1 differentiation and suppression of Th17 differentiation
Enhances antigen presentation, B-cell immunoglobulin class switching, antibody secretion, and antiviral, antiproliferative, tumor-suppressive activity
The biological activity of CTGrade GMP interleukins and growth factors is standardized, where applicable, to WHO International standards, providing cell and gene therapy developers consistent, lot-to-lot biological activity and performance.
CTGrade GMP products are manufactured in a facility that does not use or process beta-lactam containing materials. No animal- or human-derived materials were used during manufacturing or as ingredients. These products are manufactured, tested, and released in an ISO 9001:2015 certified facility following GMP. USP chapter <1043> for ancillary materials has been considered in the manufacture of these products.
SDS-PAGE Showing a Single Prominent Band at 16.9 kDa
Figure 1. CTGrade GMP rh IFN-gamma (1 μg) was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by Instant Blue staining showing a single prominent band at 16.9 kDa.
Cell-based Proliferation Assay
Figure 2. The biological activity of CTGrade GMP rh IFN-gamma was determined in a cell proliferation assay using HT-29 cells.
Lot-to-lot Consistency
Figure 3. CTGrade GMP rh IFN-gamma induces proliferation in HT-29 cells that is consistent between lots.
Bioactivity Comparison Between CTGrade GMP rh IFN-gamma and Commercially-available rh IFN-gamma
Figure 4. Bioactivity comparison between CTGrade GMP rh IFN-gamma and commercially-available rh CTGrade GMP rh IFN-gamma.
Technical Specifications
Formulation
CTGrade GMP rh IFN-gamma is lyophilized from a 0.2 μm filtered solution containing 20 mM Sodium Phosphate, 2% Trehalose, 4% Mannitol, 0.1 % Tween 80, pH 6.5
Protein Purity
≥ 98% determined by reducing and non-reducing SDS-PAGE analysis
Endotoxin
< 0.01 EU/μg using USP <85> / EP 2.6.14
Bioactivity
ED50 is determined by the dose-dependent cytotoxicity of HT-29 cells by rh IFN-gamma cells. The ED50 is typically less than 0.3 ng/mL.
Quality
Carrier-free and no animal- or human-derived materials were used during manufacturing
Specifications
Storage
This product is shipped at ambient temperature. Immediately upon receipt, store the lyophilized protein at -20°C in a manual defrost freezer.
Shelf Life
Unopened vials are stable for 36 months from the date of manufacture as indicated on the label. Reconstituted material should be apportioned in working volumes and stored at or below -20°C in manual defrost freezer. For short-term storage, reconstituted material is stable for 4-6 weeks when stored at 2-8°C. Stability can be increased by adding at least 0.1% carrier protein.
