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CTGrade GMP rh EGF is a recombinant human protein that is produced from E. coli and is designed to support preclinical and clinical research, as well as commercial applications, and offers:
High biological activity verified by a relevant bioactivity assay
Low endotoxin levels
≥ 98% purity
High lot-to-lot consistency
Size: 50 µg, 100 µg, and 1 mg. Custom sizes and packaging available on request.
EGF (epidermal growth factor) binds to the EGF receptor, a protein kinase that initiates the intracellular signaling, and is widely distributed in tissues like the kidney, cerebrum, prostate, and salivary glands.
Acts as a potent factor in promoting cell division and activating complex downstream signaling cascades
Stimulates the proliferation, differentiation, and survival of epithelial and epidermal cells
Activates at least four major downstream signaling pathways, including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLC gamma-PKC, and STAT modules
Initiates diverse cellular pathways in response to toxic environmental stimuli
For the development of advanced cell therapies, FUJIFILM Irvine Scientific is your one-stop-shop for the high-performing recombinant proteins required for expansion, proliferation, differentiation, and activation of immune and stem cells. Our CTGrade GMP recombinant proteins can be purchased separately or as a bundle with one of our high-performing PRIME-XV or classical media for a cost-effective solution.
The biological activity of CTGrade GMP interleukins and growth factors is standardized, where applicable, to WHO International standards, providing cell and gene therapy developers consistent, lot-to-lot biological activity and performance.
The CTGrade GMP products are manufactured in a facility that does not use or process beta-lactam containing materials. No animal- or human-derived materials were used during manufacturing or as ingredients. These products are manufactured, tested, and released in an ISO 9001:2015 certified facility following cGMP practices. USP chapter <1043> for ancillary materials has been considered in the manufacture of these products.
SDS-PAGE Showing Single Prominent Bands at 6.2 kDa and 6.0 kDa
Figure 1. CTGrade GMP rh EGF (1 μg) was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions, visualized by InstantBlue staining showing a single prominent band at 6.2 kDa (NR) and 6.0 kDa (R).
Cell-based Proliferation Assay
Figure 2. The biological activity of CTGrade GMP rh EGF was determined in a BALC/c 3T3 cell-based proliferation assay.
CTGrade GMP rh EGF is lyophilized from a 0.2 μm filtered solution containing 0.1% trifluoroacetic acid
Protein Purity
≥ 98% determined by reducing and non-reducing SDS-PAGE analysis
Endotoxin
≤ 0.01 EU/μg using USP <85> / EP 2.6.14
Bioactivity
ED50 is determined by the dose-dependent Proliferation of BALB/c 3T3 cells. The ED50 is typically less than 0.1 ng/mL. The international units of CTGrade GMP rh EGF is approximately 1.0 x 103 IU/µg, which is calibrated against recombinant Human Epidermal Growth Factor WHO International Standard (NIBSC code 91/530).
Quality
Carrier-free and no animal- or human-derived materials were used during manufacturing
Specifications
Storage
This product is shipped at ambient temperature. Immediately upon receipt, store the lyophilized protein at -20°C in a manual defrost freezer.
Shelf Life
Unopened vials are stable for 36 months from the date of manufacture as indicated on the label. Reconstituted material should be apportioned in working volumes and stored at or below -20°C in manual defrost freezer. For short term storage reconstituted material is stable for 4-6 weeks when stored at 2-8°C. Stability can be increased by adding at least 0.1% carrier protein.
