CultureSure Y-27632

In stock
Catalog ID
030-24021, 036-24023, 034-24024
Size Stock SKU Unit Price Qty
1 mg Inquire for Availability 030-24021
$172.00
    5 mg Inquire for Availability 036-24023
    $229.00
      25 mg Inquire for Availability 034-24024
      $831.00
        100 mg Inquire for Availability 030-24026
        $0.00
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          Details

          Y-27632 is selective and potent ROCK inhibitor. Y-27632 plays various roles including contraction of vascular smooth muscle cells via ROCK signaling pathways. Furthermore, it suppresses the cell death of human embryonic stem cells (hESCs) and human induced Pluripotent Stem Cells (hiPSCs) at cell dispersion, and that it increases the survival rate of hESCs and hiPSCs during cryopreservation.

          For Cell Culture

          Manufacturer: FUJIFILM Wako Pure Chemical Corporation
          Specification Assay: 98.0+% (HPLC)
          CAS RN: 331752-47-7
          Molecular Formula: C14H21N3O・2HCl・H2O
          Molecular Weight: 338.27
          Precautions for Use: Packed on inactive gas
          Appearance: White - pale yellow, crystalline powder - powder
          Boiling Point: about 85 degrees C
          Specific Rotation: +2 - +10 degrees (D/20 degrees C)(c=0.5,CH3OH)
          Purity: Bacterial endotoxins : < 0.25 EU/mg
          Alias: (R)-(+)-trans-N-(4-pyridyl)-4-(1-aminoethyl)-cyclohexanecarboxamide Dihydrochloride Monohydrate

           

          Preparation

          Solubility Data for Y-27632 dihydrochloride

          C14H21N3O・2HCl・H2O = 338.27

            Solvent Conc. mg/mL Conc. mmol/L
          Solubility Water 10 mg/mL 29.56 mmol/L
          Ethanol 10 mg/mL 29.56 mmol/L

          Preparing Stock Solutions

          C14H21N3O・2HCl・H2O = 338.27

          Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
          1 mmol/L 2.956 mL 14.781 mL 29.562 mL
          5 mmol/L 0.591 mL 2.956 mL 5.912 mL
          10 mmol/L 0.296 mL 1.478 mL 2.956 mL
          50 mmol/L 0.059 mL 0.296 mL 0.591 mL

           

          Y-27632 Comparison Chart

            ICH-Q7 ISO9001 Sterility
          test
          Viable
          count
          test
          Animal-
          derived-
          free
          Bacterial
          Endotoxin
          test
          Mycoplasma
          test
          Powder/
          Liquid
          References*
          Y-27632 (GMP-compliant)** ✓ ✓ - ✓ ✓ ✓ ✓ Powder -
          Y-27632, MF*** - ✓ - ✓ ✓ ✓ ✓ Powder Citations
          CultureSure Y-27632 - ✓ - - ✓ ✓ ✓ Powder Citations
          CultureSure 10mmol/L
          Y-27632 Solution,
          Animal-derived-free
          - ✓ ✓ - ✓ ✓ ✓

          Liquid

          Sterilized with filter

          Citations

           

          * Click to move to the PubMed Central search results.
          ** This product is complied with ICH-Q7 (GMP for APIs) as a raw material for commercial production of regenerative medicine products.
          *** The manufacturing process and analytical procedure are validated.

           

          Made by FUJIFILM Wako Pure Chemical Corporation and distributed by FUJIFILM Biosciences.

          • For research use only. Not for use in diagnostic procedures.
          • Product content may differ from the actual image due to minor specification changes etc.
          • If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.

          References

          1. Watanabe, K., et al.: Nat. Biotechnol., 25, 681 (2007).
            A ROCK inhibitor permits survival of dissociated human embryonic stem cells
          2. Masashi, H., et al. : Genes Dev, 1, 36(1-2), 84–102 (2022).
            Highly rigid H3.1/H3.2–H3K9me3 domains set a barrier for cell fate reprogramming in trophoblast stem cells
          3. Yoshifumi, M., et al. : J Neurosci Res, 98(8), 1575–1587 (2020).).
            Zonisamide promotes survival of human‐induced pluripotent stem cell‐derived dopaminergic neurons in the striatum of female rats
          4. Kazutoshi, T., et al. : Cell Rep Methods, 2(2), 100155 (2022).
            A stress-reduced passaging technique improves the viability of human pluripotent cells
          5. Yu, K., et al. : eNeuro, 9(2), ENEURO.0442-21 (2022).
            Characterization of Hypothalamic MCH Neuron Development in a 3D Differentiation System of Mouse Embryonic Stem Cells
          6. Tomoya, U., et al. : Cell Rep Med, 2(6) 100298 (2021).
            A muscle fatigue-like contractile decline was recapitulated using skeletal myotubes from Duchenne muscular dystrophy patient-derived iPSCs
          7. Genki, N., et al. : eLife, 11, e77007 (2022).
            Robotic search for optimal cell culture in regenerative medicine
          8. Maobi, Z., et al. : Cell, 8(8), 933 (2019).
            Estrogen and EGFR Pathways Regulate Notch Signaling in Opposing Directions for Multi-Ciliogenesis in the Fallopian Tube
          9. Yu, K., et al. : Front Endocrinol (Lausanne), 13, 941166 (2022).
            EpCAM Is a Surface Marker for Enriching Anterior Pituitary Cells From Human Hypothalamic-Pituitary Organoids
          10. Sho, T., et al. : STAR Protoc, 3(2), 101360 (2022).
            Purification of cardiomyocytes and neurons derived from human pluripotent stem cells by inhibition of de novo fatty acid synthesis
          11. Takuro, T., et al. : Commun Biol, 5, 453, (2022).
            Extremely low-frequency pulses of faint magnetic field induce mitophagy to rejuvenate mitochondria
          12. Tomoki, Y., et al. : Mol Ther Methods Clin Dev, 10(22), 263–278 (2021).
            Monolayer platform using human biopsy-derived duodenal organoids for pharmaceutical research
          13. Bin, X., et al. : J Neurochem, 153(6), e14949 (2020).
            Transplantation of iPS‐derived vascular endothelial cells improves white matter ischemic damage
          14. Hiroyuki, N., et al. : Front Physiol, 10, 818 (2019).
            Geometrical Patterning and Constituent Cell Heterogeneity Facilitate Electrical Conduction Disturbances in a Human Induced Pluripotent Stem Cell-Based Platform: An In vitro Disease Model of Atrial Arrhythmias
          15. Yayoi, H., et al. : Front Pharmacol, 12, 680618 (2021).
            High-Throughput Drug Screening System Based on Human Induced Pluripotent Stem Cell-Derived Atrial Myocytes ∼ A Novel Platform to Detect Cardiac Toxicity for Atrial Arrhythmias
          16. Masahiko, A., et al. : Nat Commun, 12, 4507 (2021).
            Therapeutic manipulation of IKBKAP mis-splicing with a small molecule to cure familial dysautonomia
          17. Maobi, Z., et al. : Biomolecules, 10(9), 1270 (2020).
            Fallopian Tube Basal Stem Cells Reproducing the Epithelial Sheets In Vitro—Stem Cell of Fallopian Epithelium
          18. Junko, Y., et al. : iScience, 25(7), 104538 (2022).
            StemPanTox: A fast and wide-target drug assessment system for tailor-made safety evaluations using personalized iPS cells
          19. Keitaro, K., et al. : J Endocr Soc, 3(11), 1969–1978 (2019).
            Pathogenesis of Anti–PIT-1 Antibody Syndrome: PIT-1 Presentation by HLA Class I on Anterior Pituitary Cells
          20. Koichiro, O., et al. :Sci Rep, 8, 3615 (2018).
            Vasopressin-secreting neurons derived from human embryonic stem cells through specific induction of dorsal hypothalamic progenitors
          21. Kengo, K., et al. : Sci Rep, 11, 22476 (2021)
            Requirement of direct contact between chondrocytes and macrophages for the maturation of regenerative cartilage
          22. Hayato, F., et al. : J Endocr Soc, 3(11), 1969–1978 (2019).
            Alpha‐synuclein dynamics in induced pluripotent stem cell‐derived dopaminergic neurons from a Parkinson’s disease patient (PARK4) with SNCA triplication
          23. Yoji, K., et al. : Life Sci Alliance, 4(5), e202000974 (2021).
            GATA transcription factors, SOX17 and TFAP2C, drive the human germ-cell specification program
          24. Eri, N., et al. : Biochem Biophys Rep, 29, 101195 (2022).
            Slow diffusion on the monolayer culture enhances auto/paracrine effects of Noggin in differentiation of human iPS cells induced by BMP
          25. Kun, H., et al. : Sci Rep, 10, 2558 (2020).
            Inhibition of cyclooxygenase-1 by nonsteroidal anti-inflammatory drugs demethylates MeR2 enhancer and promotes Mbnl1 transcription in myogenic cells
          26. Yusuke, T., et al. : Int J Mol Sci, 20(6), 1331 (2019).
            ROCK2 Regulates Monocyte Migration and Cell to Cell Adhesion in Vascular Endothelial Cells

          Specifications

          Storage
          Keep at -20°C
          Product Sheets
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