Q: What is the antigen?

A: It is a synthetic peptide of P2RY12 (corresponding to the C-terminus). The specific sequence is not disclosed.

Q: What secondary antibodies can be used?

A: At Fujifilm Wako, the following secondary antibodies have been successfully used:

• Alexa Fluor 488 AffiniPure Goat Anti-Guinea Pig IgG (H+L) (Jackson ImmunoResearch, Product Number: 106-545-003)
• Alexa Fluor 488 AffiniPure Donkey Anti-Guinea Pig IgG (H+L) (Jackson ImmunoResearch, Product Number: 706-545-148)
• Alexa Fluor 647 AffiniPure Donkey Anti-Guinea Pig IgG (H+L) (Jackson ImmunoResearch, Product Number: 706-605-148)

Q: What animals can this antibody be used for?

A: Fujifilm Wako has confirmed that it can be used for mice. There are also reports of staining in rats, although we have no experience of this at our company. For details, please refer to the “Data” section. Other species have not been tested.

Q: What kind of tissues or sites can be stained?

A: Fujifilm Wako have experience in staining the following tissue and site:

• Cerebral cortex
• Hippocampus
• Hypothalamus
• Cerebellum
• Olfactory bulb
• Spinal cord

There are also reports of staining results for the following tissues and sites. For details, please refer to the “Data” on this page.Prefrontal cortex

• Medulla oblongata area postrema
• Median eminence
• Retina

Q: Can microglia other than homeostatic microglia be stained?

A: P2RY12 is a marker for homeostatic microglia, and it is known that the expression level of P2RY12 is reduced in disease-associated microglia (DAM) and microglia in Alzheimer's disease model mice2-3). In these microglia, It is possible that these microglia cannot be stained due to the reduced expression.

Q: The background of immunohistochemistry is high.

A: Try glycine treatment (In 25 mM glycine/PBS for about 20 minutes) after preparation of frozen sections. It is possible to inactivate the aldehyde groups in the sample and suppress the background.