Anti Iba1, Rabbit Monoclonal Antibody (6A4), recombinant

Iba1 (Ionized calcium-binding adapter molecule 1) is an approximately 17 kDa calcium-binding protein. It is used as a microglial marker because it is expressed specifically in microglia in the central nervous system1). It is expressed in both resting and activated microglia, but is reportedly expressed more highly in activated microglia2). It is also expressed in macrophages in peripheral tissues and is known as AIF-1 (Allograft inflammatory factor-1). Iba1 binds to F-actin in cells to form actin bundles. The formation of actin bundles is thought to be required for the membrane ruffling observed during cell migration and phagocytosis3).

Anti Iba1, Rabbit Monoclonal Antibody (6A4), recombinant is a rabbit monoclonal antibody that has the same performance as "Anti Iba1, Rabbit (for immunocytochemistry)" (Product Number 019-19741) in immunohistochemical staining of mouse and rat brains. It has also been reported to obtain good results in immunohistochemical staining of mouse retinas.

• Enables even the processes of microglia to be immunohistochemical stained.
• Same performance as "Anti Iba1, Rabbit (for immunocytochemistry)".
• Relatively small amount needed, with concentrations ranging from 1:200 to 1:10000.
• Can be used for immunohistochemical staining of mouse retinas.

For Immunochemistry

Made by FUJIFILM Wako Pure Chemical Corporation and distributed by FUJIFILM Irvine Scientific.

• For research use only. Not for use in diagnostic procedures.

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1. Imai, Y., Ibata, I., Ito, D., Ohsawa, K., & Kohsaka, S.: Biochem. Biophys. Res. Commun., 224(3), 855(1996).
   A Novel Geneiba1 in the Major Histocompatibility Complex Class III Region Encoding an EF Hand Protein Expressed in a Monocytic Lineage
2. Mori, I., Imai, Y., Kohsaka, S., & Kimura, Y.: Microbiol. Immunol., 44(8), 729(2000).
   Upregulated expression of Iba1 molecules in the central nervous system of mice in response to neurovirulent influenza A virus infection
3. Sasaki, Y., Ohsawa, K., Kanazawa, H., Kohsaka, S., & Imai, Y. Biochem. Biophys. Res. Commun., 286(2), 292(2001).
   Iba1 is an actin-cross-linking protein in macrophages/microglia.