Anti CD9, Rat Monoclonal Antibody (30B), Biotin Conjugated Application Data

Exosomes in biofluids were detected using PS Capture Exosome ELISA Kit (Streptavidin HRP) (Catalog ID: 298-80601). Anti CD9, Rat Monoclonal Antibody (30B), Biotin Conjugated (Catalog ID: 019-27953) was used as a primary antibody. Then, HRP-conjugated Streptavidin and TMB solution in the ELSIA kit were used for detection.

Sample	Human serum or human heparin plasma
Primary antibody	Anti CD9, Rat Monoclonal Antibody (30B), Biotin Conjugated (Catalog ID: 019-27953) (1:4,000)
Detection	HRP-conjugated streptavidin and TMB solution in the ELISA kit

Exosomes in human serum and human heparin plasma were detected concentration-dependently with Anti CD9, Rat Monoclonal Antibody (30B), Biotin Conjugated (Catalog ID: 019-27953).

Exosomes in cell culture supernatant of COLO201 cells were detected using PS Capture Exosome ELISA Kit (Streptavidin HRP) (Catalog ID: 298-80601). As primary antibodies, Anti CD9, Rat Monoclonal Antibody (30B), Biotin Conjugated (Catalog ID: 019-27953) and three competitive anti CD9 antibodies (biotin conjugated) were used. Then, HRP-conjugated Streptavidin and TMB solution in the ELSIA kit were used for detection.

Sample	Cell culture supernatant of COLO201
Primary antibody	Anti CD9, Rat Monoclonal Antibody (30B), Biotin Conjugated (Catalog ID: 019-27953) (1:4,000) and competitive biotin conjugated antibodies
Detection	HRP-conjugated streptavidin and TMB solution in the ELISA kit

CD9 proteins on exosomes isolated using MagCapture Exosome Isolation Kit PS (Product Code: 293-77601) were detected with Anti CD9, Rat Monoclonal Antibody (30B), Biotin Conjugated (Catalog ID: 019-27953) using a Western Blot method in non-reducing conditions.

Sample	Exosomes from human serum, human heparin plasma and cell culture supernatant of COLO201
Gel	SuperSep Ace, 10-20%, 17well (Product code: 198-15041)
Primary antibody	Anti CD9, Rat Monoclonal Antibody (30B), Biotin Conjugated (Catalog ID: 019-27953) (1:1,000)
Detection	HRP-Streptavidin and ImmunoStar Zeta (Product Code: 295-72404)