Anti Iba1, Rabbit Monoclonal Antibody (6A4), recombinant Data

Performance Data

Comparison with our conventional product (rabbit polyclonal antibody)

Polyclonal Antibody
(Product No. 019-19741)

Monoclonal Antibody
(Product No. 018-28523)

Species: Rat
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1:200

Data by courtesy of
Dr. Nakajima, Faculty of Science and Engineering, Soka University

Comparison with company A (rabbit monoclonal antibody)

Company A
(rabbit, monoclonal)

Fujifilm Wako
(Product No. 018-28523)

Species: Mouse
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1:1,000

Application Data

Immunohistochemistry

Mouse

Species: Mouse
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1:1,000

Species: Mouse
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1:200

Data by courtesy of
Dr. Koizumi, Interdisciplinary Graduate School of Medicine, University of Yamanashi

Species: Mouse
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1:200
Note: Preparation of frozen sections → PFA fixation

Data by courtesy of
Dr. Ino, Graduate School of Medicine, Osaka University

Species: Mouse
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1:1,000

Data by courtesy of
Dr. Nonaka, Graduate School of Engineering, Kyoto University

Species: Mouse
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1:1,000

Data by courtesy of
Dr. Ito, Graduate School of Medicine, Juntendo University

Species: Mouse
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1:1,000

Data by courtesy of
Dr. Tsuruta and Mr. Ueda, Faculty of Life and Environmental Sciences, University of Tsukuba

Species: Mouse
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1:1,000

Data by courtesy of
Dr. O, S University

Species: Mouse
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1:1,000

Data by courtesy of
Dr. Y, Institute of R

Species: Mouse (APP^NL-F/NL-F)
Site: Cerebrum
Sample: Fixed tissue slice
Antibody concentration: 1:200
Note: Tissue clearing with Scale method

Data by courtesy of
Dr. Hama, RIKEN Center for Brain Science

Species: Mouse (Model of Alzheimer Disease)
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1:500

Data by courtesy of
Dr. Konishi, Graduate School of Medicine, Nagoya University

Species: Mouse (Model of Focal Stroke)
Site: Cerebral cortex
Sample: Paraffin section
Antibody concentration: 1:500

Data by courtesy of
Dr. M, C University

Species: Mouse (Model of neuronal ceroid lipofuscinoses)
Site: Cerebral cortex
Sample: Paraffin section
Antibody concentration: 1:500

Data by courtesy of
Dr. T and S, J University

Species: Mouse
Site: Hippocampus
Sample: Frozen section
Antibody concentration: 1:1,000

Data by courtesy of
Dr. F, A University

Species: Mouse (APPNL-G-F)
Site: Hippocampus
Sample: Frozen section
Antibody concentration: 1:1,000

Data by courtesy of
Dr. Takatori, Graduate School of Pharmaceutical Sciences, University of Tokyo

Species: Mouse
Site: Brainstem (Principal sensory trigeminal nucleus)
Sample: Vibratome section
Antibody concentration: 1:500
Note: Tissue clearing with Scale method

Data by courtesy of
Dr. Ueta, School of Medicine,
Tokyo Women's Medical University

Species: Mouse
Site: Retina (Post-surgery)
Sample: Frozen section
Antibody concentration: 1:500

Data by courtesy of
Dr. Hayashi, School of Medicine, Aichi Medical University

Species: Mouse
Site: Retina (Flat-mount)
Sample: Frozen section
Antibody concentration: 1:2,000

Data by courtesy of
Dr. Watanabe, Dr. Iwagawa, University of Tokyo Hospital

Species: Mouse
Site: Retina (Flat-mount)
Antibody concentration: 1:1,000

Data by courtesy of
Dr. Watanabe, Dr. Iwagawa, University of Tokyo Hospital

Normal

Activated

Species: Mouse
Site: Retina (Cross-section)
Sample: Frozen section
Antibody concentration: 1:2,000

Data by courtesy of
Dr. Watanabe, Dr. Iwagawa, University of Tokyo Hospital

Species: Mouse
Site: Spinal cord
Sample: Frozen section
Antibody concentration: 1:2,000

Data by courtesy of
Dr. Hagiwara and Mr. Sawada, Faculty of Science and Technology, Tokyo University of Science

Species: Mouse
Site: Lumbar spinal cord
Sample: Frozen section
Antibody concentration: 1:1,200

Data by courtesy of
Dr. Takase, Graduate School of Medical Sciences, Kyushu University

Species: Mouse
Site: Lung
Sample: Paraffin section
Antibody concentration: 1:1,000

Data by courtesy of
Dr. K, N University

Rat

Species: Rat
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1:1,000

Species: Rat
Site: Cerebral cortex (Medial prefrontal cortex)
Sample: Frozen section
Antibody concentration: 1:1,000

Data by courtesy of
Dr. Ohta, Faculty of Medicine, Kagawa University

Immunocytochemistry

Cell line: SIM-A9
Antibody concentration: 1:200

Data by courtesy of
Dr. I, University of T

The data shown above are courtesy of the researchers and do not guarantee experimental results.
Please note that Fujifilm Wako cannot answer any questions regarding the data above.

Flow Cytometry

The microglial cell line BV-2 cells were fixed and permeabilized using the BD Cytofix/Cytoperm™ Fixation/Permeabilization Kit (BD Biosciences, 554714). Cells were then stained with either buffer, isotype control, or this product, followed by staining with the PE-labeled secondary antibody (SouthernBiotech, 4030-09). The fluorescence-labeled cells were analyzed by flow cytometry.

Result: Fixed and permeabilized BV-2 cells were successfully detected using the anti Iba1 antibody and flow cytometry.

User Voice

Researchers evaluated this antibody during the "Anti-Iba1 Rabbit Monoclonal Antibody Free Sample Campaign" held from March to May 2023 in Japan. Below are the results of the survey submitted by the researchers.