DNA or RNA solution (100 µl)
↓←3.3 µl, 3 mol/l Sodium Acetate(Product Component) ^*1
↓←1 µl, Ethachinmate ^*2
↓vortex ^*3
↓←200 - 250 µl, Ethanol
↓vortex ^*3
↓12 K x g for 5min at room temperature ^*4
pellet ^*5

*1 Final salt concentration must be more than 0.1 mol/l.
*2 Add 1 µl of Ethachinmate per 100 µl of DNA solution. If the amount of DNA solution is less than 100 µl, add 1 µl of Ethachinmate. If the amount of DNA solution is more than 300 µl, 3 µl of Ethachinmate is the correct amount to be added. Once Ethachinmate is added into DNA solution, there is no need to add Etachinmate again if performing more centrifugations. Adding to much Ethachinmate may make the solution viscous, causing the following processes to be difficult.
*3 Vortexing improves the efficiency to recover subtle DNA.
*4 Cooling is not necessary.
*5 Pellet is visible. Pellet may then be dissolved in buffer and used as a template for enzyme reactions. Wash with 70 % ethanol, if needed.

When total RNA is extracted using ISOGEN, the RNA pellet ma sometimes be difficult to see.
By adding Ethachinmate, the pellet is easier to see.

Sample
↓←ISOGEN
↓Homogenize and incubate at room temperature for 5min.
↓←Chloroform
↓Shake vigorously and incubate at room temperature for 3min.
↓Centrifuge for 15 min to separate the phases
↓Transfer the aqueous phase to a new tube
↓←1-2 µl of Ethachinmate ^*1
↓vortex
↓←Isopropanol.
↓Mix by gentle inversion and incubate at room temperature for 10 min.
↓Centrifuge for 10 min.
↓Remove the supernatant.
Pellet
↓←70 % Ethanol
↓vortex
↓Centrifuge for 5 min.
↓Remove the supernatant
Pellet
↓Air-dry the pellet.
↓←RNase-free water or TE buffer (pH 8.0) for dissolving the pellet.
Total RNA Solution